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. 2009 Aug 3;206(8):1787–1802. doi: 10.1084/jem.20090641

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© 2009 Pilichou et al.

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Figure 9. — Immunohistochemical analysis of cardiac cryosections from 2–3-wk-old mice injected with EBD. (A and B) WT and Tg-WT hearts show normal distribution of desmin, dystrophin, and pan-cadherin in the ventricles, and no EBD-positive cells. Bars, 250 µm. (C and D) In contrast, hearts from Tg-NS mice display large areas of ventricular myocardium lacking desmin, dystrophin, and pan-cadherin. These abnormal areas contained myocytes positive for EBD, indicating increased cell membrane permeability and loss of myocyte integrity and viability. Bars: (C) 250 µm; (D) 50 µm.