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. 2009 Aug 3;206(8):1803–1816. doi: 10.1084/jem.20082815

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© 2009 Cadera et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Markers of receptor editing are increased in IκBα-expressing cells. (A) The top diagram shows that the κ locus is in its germline configuration with the primary break intermediate shown below. The bottom diagram is the κ locus after a Vκ has rearranged to Jκ1, with the secondary break intermediate shown below. (B) Agarose gel analysis of PCR assays for RS rearrangements to either IRS (RS-IRS) or Vκ (RS-Vκ) in DNA from pre–B cells sorted for β-gal expression. PCR amplification of the APRT locus was used as a template control, and H₂0 indicates PCR reactions without template. (C) LM-PCR assays to detect primary and secondary double-stranded DNA RSS break intermediates in DNA from sorted β-gal–positive and –negative pre–B cells. An ethidium-stained agarose gel analysis of LM-PCR products is shown. Pooled bone marrow from five to six mice was used for each of two repetitions of this experiment yielding similar results.