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. 2009 Aug 3;206(8):1803–1816. doi: 10.1084/jem.20082815

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© 2009 Cadera et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Expression of NF-κB target genes in IκBαΔ-transduced pre–B cells. (A) cDNA from sorted β-gal–positive and –negative pre–B cells was analyzed by real time RT-PCR for IRF4 transcripts and the data were normalized to HPRT transcripts to control for any differences in cDNA template. β-gal–negative cell result was arbitrarily set to 1, with error bars indicating range of triplicate assays. (B) cDNA from CD19⁺IgD⁻ cells from B1-8 α-HEL-κ and B1-8^low α-HEL-κ mice was analyzed by real time RT-PCR for IRF4 transcripts and the data were normalized to HPRT, with error bars indicating range of triplicate assays. These results are representative of two independent repetitions of this experiment using pools of sorted bone marrow cells from five to six mice.