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. 2009 Aug 3;206(8):1681–1690. doi: 10.1084/jem.20082462

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© 2009 The Rockefeller University Press

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Figure 4. — The Th2-polarizing function of both SEA and omega-1 is sensitive to ribonuclease inhibition. (A and B) Equal amounts of DEPC-treated or -untreated unfractionated SEA (40 µg/ml) or purified omega-1 (0.5 µg/ml) were added to the DC/DO.11.10 Tg CD4 T cell cultures, and IL-4 secretion was measured on day 3 by ELISA (A) and the frequency of IL-4⁺ cells was assayed by ICS on day 7 after restimulation with anti-CD3 mAb (B). Bars represent means ± SD for IL-4. The dot plots shown are gated on CD4⁺ T lymphocytes (percentages are shown). When DEPC-treated SEA or DEPC-treated omega-1 was added to the cultures in the presence of exogenous rIL-4, to evaluate possible nonspecific inhibitory effects of carryover DEPC, no decrease in the frequency of IL-4⁺ DO.11.10⁺ T cells was observed (not depicted). The data shown are representative of two experiments performed.