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. 2000 Nov 14;97(24):13318–13323. doi: 10.1073/pnas.230453597

Figure 2.

Figure 2

Intrahepatic inoculation of chimpanzee 1590 with RNA transcripts of pH77C (ΔHVR1). Serum samples were collected weekly from the chimpanzee and monitored for HCV-RNA (In-house RT-nested PCR and HCV Monitor test version 2.0; Roche), HCV antibodies (second generation ELISA, Abbott), and liver enzyme levels (alanine aminotransferase, Anilytics). PBMC were collected weekly and tested for HCV-specific proliferative capacity (Peripheral CD4) with a panel of recombinant HCV proteins [C22 (core), C33-c (NS3), C100 (NS3-NS4), and NS5]. The peripheral CTL was tested by stimulating PBMC with a large panel of HCV peptides corresponding to known CTL epitopes. Expanded T cells isolated from liver biopsy samples were tested for HCV-specific proliferative responses (Intrahepatic CD4). +, positive; −, negative. Liver biopsies were examined also for necroinflammatory changes (0, normal; 1+, 2+, 3+, 4+).