Table 2.
Changes in the different parameters indicating oxidative stress status in rats treated with aflatoxinB1 and phytic acid
| Treatment period | Recovery period | |||||
|---|---|---|---|---|---|---|
| 3 days | 7 days | 14 days | 21 days | 28 days | ||
| Testis lipid peroxidation (nmol MDA/100 mg/h) | Control | 58.82 ± 5.32b | 59.53 ± 4.47b | 56.13 ± 2.82bc | 59.78 ± 5.32b | 55.89 ± 9.45a |
| AFB1 | 90.63 ± 9.23b | 93.67 ± 16.97a | 83.84 ± ± 9.57a | 77.65 ± 6.87a | 57.54 ± 5.10a | |
| Phytic acid | 69.32 ± ± 5.92b | 53.32 ± 8.34b | 46.06 ± 7.54c | 58.74 ± 4.45b | 39.90 ± 4.35b | |
| AFB1+Phytic | 56.55 ± 6.0b | 69.93 ± 11.28b | 61.11 ± 2.94b | 60.98 ± 7.98b | 46.21 ± 6.20ab | |
| Total thiols (nmol/100 mg tissue) | Control | 174.29 ± 11.64a | 186.75 ± 10.10a | 189.11 ± 4.42b | 180.634 ± 7.43c | 187.28 ± 11.62 |
| AFB1 | 126.49 ± 15.62b | 124.77 ± 13.58c | 174.17 ± 5.73c | 177.543 ± 4.87c | 198.77 ± 16.11 | |
| Phytic acid | 181.59 ± 14.10a | 205.45 ± 13.06a | 208.33 ± 9.22a | 211.897 ± 6.43a | 207.99 ± 20.70 | |
| AFB1+Phytic | 183.81 ± 17.75a | 158.25 ± 7.96b | 188.12 ± 10.44b | 195.312 ± 6.23b | 187.99 ± 12.86 | |
| Testis glutathione (nmol/100 mg tissue) | Control | 45.471 ± 4.150 a | 51.239 ± 3.604 a | 43.687 ± 4.012 b | 53.707 ± 5.292 | 52.402 ± 3.775 |
| AFB1 | 20.931 ± ± 5.975 c | 27.386 ± 6.409 b | 29.818 ± 4.308 c | 38.020 ± 2.537 | 45.706 ± 4.644 | |
| Phytic acid | 48.268 ± 6.123 a | 56.374 ± 2.800a | 53.254 ± 2.635 a | 48.570 ± 8.150 | 49.507 ± 6.863 | |
| AFB1+Phytic | 33.048 ± 4.135 b | 35.255 ± 4.362 b | 42.684 ± 5.097 b | 43.059 ± 6.963 | 40.795 ± 5.247 | |
| Total peroxidase (absorbance) | Control | 0.112 ± 0.006a | 0.101 ± 0.016 | 0.107 ± 0.012 | 0.110 ± 0.054b | 0.107 ± 0.070 |
| AFB1 | 0.067 ± 0.112b | 0.086 ± 0.034 | 0.115 ± 0.017 | 0.100 ± 0.033b | 0.111 ± 0.010 | |
| Phytic acid | 0.129 ± 0.003a | 0.104 ± 0.039 | 0.116 ± 0.012 | 0.120 ± 0.067a | 0.087 ± 0.011 | |
| AFB1+Phytic | 0.110 ± 0.011a | 0.123 ± 0.022 | 0.098 ± 0.010 | 0.120 ± 0.092a | 0.123 ± 0.018 | |
| Superoxide dismutase (U/100 mg tissue) | Control | 20.83 ± 3.175ab | 18.67 ± 2.254 | 21.77 ± 2.411 | 21.47 ± 1.1985a | 20.50 ± 3.970 |
| AFB1 | 13.00 ± 1.513c | 13.81 ± 2.108 | 16.20 ± 2.654 | 16.85 ± 0.1729b | 21.43 ± 1.795 | |
| Phytic acid | 17.87 ± 1.436b | 16.80 ± 2.402 | 23.63 ± 4.290 | 20.80 ± 1.417a | 22.60 ± 1.566 | |
| AFB1+Phytic | 33.88 ± 2.804a | 19.00 ± 2.000 | 22.76 ± 2.112 | 19.70 ± 0.821ab | 23.63 ± 2.500 | |
| Testis catalase (k ×10−2) | Control | 19.593 ± 0.697 a | 20.627 ± 1.585 a | 23.634 ± 4.216 a | 23.559 ± 2.405 a | 22.723 ± 3.252 |
| AFB1 | 11.799 ± 3.090 b | 12.876 ± 2.142 c | 14.631 ± 1.065 b | 15.247 ± 2.002b | 18.492 ± 3.983 | |
| Phytic acid | 17.927 ± 1.364 a | 20.920 ± 1.563 a | 18.803 ± 1.634 ab | 20.464 ± 2.856 ab | 20.476 ± 1.841 | |
| AFB1+Phytic | 12.785 ± 2.502 b | 17.075 ± 1.293 b | 18.120 ± 1.678 b | 18.267 ± 4.153 ab | 20.519 ± 5.353 | |
Number of animals = 6; Values are expressed as Means ±SD; AFB1, aflatoxinB1; Means within a row with the same letter are not significantly different (P > 0.05).