Figure 1.

Generation of gene-targeted mice. (A) Targeting construct for the generation of floxed Cypher mice. In the targeting vector, cypher gene exon 1 is flanked by LoxP sites as indicated with a black triangle. The neomyosin resistant gene is surrounded by FLP cassettes as shown by black rectangles. (B) Southern blot analysis shows a 6.9 kb band in heterozyogous gene-targeted embryonic stem cells when compared with a 6.2 kb band in the wild-type allele. (C) Representative western blot results showing the effective deletion of Cypher in CKO mouse heart at 1 month of age.