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. 2008 Nov 21;18(4):701–713. doi: 10.1093/hmg/ddn400

Figure 4.

Figure 4.

Inducible cardiac-specific deletion of Cypher. (A) Western blot results showing inducible deletion of Cypher in the heart of Cypherf/f/Cre mice 1 month after injection with tamoxifen [ICKO (inducible cardiac-specific CKO)] or Cypherf/f/Cre and Cypherf/f littermate mouse injected with peanut oil (Con/P) and tamoxifen (Con/T), respectively, as controls. (B) Accumulative survival curve showing that ICKO mice die from 1 week after tamoxifen injection, and all die before 15 weeks after injection, compared with 100% survival of both the control groups (n = 27 for ICKO group, n = 20 for tamoxifen control group and n = 22 for peanut oil control group). (C) Hematoxylin and eosin staining showing severe dilated ventricules in ICKO mouse heart 1 month after injection, compared with tamoxifen control mouse heart (Con/T). Echocardiography data showing (D) LVIDd, (E) LVIDs, (F) LVPWs and (G) FS results in ICKO and control littermate mice 1 month after tamoxifen or peanut oil injection (n = 4 for each group). LVIDd, end-diastolic left ventricular internal dimension; LVIDs, end-systolic left ventricular internal dimension; LVPWs, systolic left ventricular posterior wall thickness; % FS, percentage of fractional shortening; #P < 0.01.