Figure 5.

PGC-1α/β expression increases the levels of assembled OXPHOS complexes but does not change the relative fraction incorporated into supercomplexes. Cell samples were solubilized in either lauryl maltoside (A) or digitonin (B) to preserve the complex or supercomplex structure, respectively. Samples were separated in a 4–16% PAGE as described in Materials and Methods, transferred to a PVDF membrane and probed with antibodies representative of OXPHOS complexes. COXI (complex IV), core-2 (complex III), ND39 (complex I), VDAC1 and tubulin.