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. 2009 Mar 9;18(10):1825–1838. doi: 10.1093/hmg/ddp099

Figure 8.

Figure 8.

SUT-2 physically interacts with ZYG-12. (A) In vitro confirmation of the yeast two hybrid interaction between SUT-2 and ZYG-12. Radiolabled ZYG-12 protein was tested against immobilized recombinant GST–SUT-2 fusion protein or recombinant GST alone using GST pull-down assays. (B) SUT-2 binds both N- and C-terminal domains containing fragments of ZYG-12 in GST pull-down assays. T is a single pass transmembrane domain. (C) A radiolabled SUT-2 protein fragment containing the S2NH domain is sufficient for binding to immobilized ZYG-12. (D) Loss of zyg-12 causes upregulation of SUT-2 protein. SUT-2 80 kDa band is ∼3-fold more abundant in zyg-12(bw54) than in N2.