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Western blot analysis of P19 cells transfected with wild-type and mutant NODAL constructs. (A) Expression of phosphorylated Smad2 and Smad2 in P19 cells transfected with either wild-type or mutant NODAL. β-Actin serves as a loading control. (B) Quantitation of western blots for phosphorylated Smad2 versus Smad2 in cells treated with either wild-type or mutant NODAL expression vectors. c700indel, c.700_723delinsTTGACTTCC.
