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Importance of monitoring transition ions when conducting targeted quantitative studies. The inset shows the tandem mass spectrometry (MS2) spectrum of the HER-2 peptide SGGGDLTLGLEPSEEEAPR. The bottom four spectra show peaks throughout the chromatograms when the parent ion and three transition ions labeled in the MS2 spectrum are monitored. Collectively presented spectra show that the peak at ∼31 min (asterisk and outlined) is the peptide of interest.
