Figure 2:

Effect of matrix concentration on targeting specific peptide in complex mixture using MS. Increasing amounts of cell lysates was added to a peptide internal standard and the MS signals obtained using MRM. As the amount of matrix added increased, a concomitant decrease in the area of the peaks corresponding to the transition ions (open circle) m/z 924.5, (filled triangle) m/z 1053.5, (open triangle) m/z 1224.5, as well as their total areas (filled circle) was observed.