Skip to main content
. 2009 Jul 23;106(31):12706–12711. doi: 10.1073/pnas.0905046106

Fig. 2.

Fig. 2.

Representative force-extension curves and summary of mechanical unfolding results. Red and black curves are for pulling and relaxing, respectively, with waiting time at approximately 3 pN for 0 or 10 s. (A–D) Representative force-extension curves of ΔU177. (E–G) Representative force-extension curves of mutant CCC (with 3 major-groove U·A-U base triples disrupted, see Fig. 1B). (H) Summary of unfolding force-versus-extension change of ΔU177. (I) Summary of unfolding force-versus-extension change of mutant CCC. (J) A force-extension curve of ΔU177 folding intermediate structure with low-force 2-step unfolding. (K) A force-extension curve of isolated stem 1 hairpin derived from ΔU177 (see Fig. S4 A). (L) A force-extension curve of isolated stem 2 hairpin derived from ΔU177. (M) Unfolding force histogram of the second step of low-force 2-step unfolding of the folding intermediate structure of pseudoknot ΔU177. (N) Unfolding force histogram of isolated stem 1 hairpin derived from ΔU177 (see Fig. S4 B). (O) Unfolding force histogram of isolated stem 2 hairpin derived from ΔU177.