Confocal images of transformed L. amoebiformis cells, with GFP fluorescence. The images labeled GFP show GFP localization (green), and those labeled plastids show chlorophyll-autofluorescence (red). (A) A cell transformed with pBnAtpD62+GFP showing the GFP localization in the stromas and pyrenoids of plastids. (B) A cell transformed with pGFP-Only (a control), showing the GFP localization in the nucleus and cytoplasm. (C) A cell transformed with pBnAtpD30+GFP, showing the GFP localization in the putative ER. (D) A cell transformed with pLaCRT400+GFP, showing the GFP in the ER. (Scale bar, 5 μm.) DIC, differential interference contrast; Cy, cytoplasm; N, nucleus; PS, plastid stroma; Py, pyrenoid; MP, mature protein.