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In vivo targeting of GFP fused with BnAtpD preproteins possessing various substitutions in the TPL. (A) Amino acid sequences of 12 constructs used for the substitution analyses. Characters in blue indicate the alanine residues substituted at the positions of basic amino acids (red characters). Red arrowheads indicate the predicted TPL cleavage site. (B) In vivo targeting-efficiencies of GFP fusion proteins when cells were transformed with each plasmid construct.
