Fig. 5.

Confirmation of functional compatibility of bipartite plastid-targeting peptides among the chlorarachniophytes, cryptophytes, and apicomplexans. Confocal images of transformed cells expressing heterologous plastid preproteins fused with GFP. (A) Localization of GFP fused with acyl carrier protein of T. gondii (TgAcp183+GFP) in a L. amoebiformis cell. (B) Localization of GFP fused with light harvesting complex protein of G. theta (GtLhcp233+GFP) in a L. amoebiformis cell. (C) Localization of GFP fused with an ATP synthase delta subunit of B. natans (BnAtpD247+GFP) in T. gondii cells. (D) Localization of the GtLhcp233+GFP protein in T. gondii cells. To detect apicoplasts in T. gondii cells, DsRed (red fluorescent protein) fused with an apicoplast-targeted preprotein of T. gondii (TgAcp183+DsRed) was used. AP, apicoplast. (Scale bar, 5 μm.)