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. 2009 Jul 20;106(31):13106–13111. doi: 10.1073/pnas.0906250106

Fig. 2.

Fig. 2.

Cp-actin filament-dependent chloroplast motility. (A) Cp-actin filaments disappearance under high-intensity blue light before (Upper) and 5 min after (Lower) microbeam irradiation. (D) Cp-actin filaments increase under low-intensity blue light before (Upper) and 10 min after (Lower) microbeam irradiation. Microbeam, 30 μm in width as indicated by brackets and red dotted lines. (B and E) Changes in the relative amount of cp-actin filaments (based on fluorescence intensity) before and after blue light irradiation. (C and F) Motility of chloroplasts located inside and outside the microbeam. The integrated distance traveled for 60 min under high- or low-intensity light was determined. Data in B, C, E, and F indicate a mean and SEs of 9 chloroplasts derived from 3 independent experiments, respectively. (G) The absence of cp-actin filaments in the chup1 mutant. (Inset) High magnification of a chloroplast without cp-actin filments. (H) Chloroplast motility along cytoplasmic streaming is high in chup1 mutants (Movie S9) compared with WT. Each colored curve represents an independent chloroplast.