avrBs2-dependent localized cell death in resistant
Bs2 pepper is mediated via an mRNA signal.
(A) Xcv symptoms on resistant
Bs2 pepper leaves. Plants were inoculated with a 2
× 108 cells ml−1 suspension of
Xcv strain GM98–38 carrying pDD62 (empty vector),
pDD62(avrBs21–714),
pVSP61(avrBs219–714-HA),
pVSP61(avrBs2+1fs-HA), or
pVSP61(avrBs2+2fs-HA). Symptoms were
photographed 48 h after inoculation. (B)
Agrobacterium-mediated transient expression of
avrBs2 genes in resistant Bs2 pepper
leaves. Plants were inoculated with a 6 × 108 cells
ml-1 suspension of A. tumefaciens
(Agro) strain C58C1 (pCH32) carrying pMD1 (empty
vector), pMD1(avrBs21–714),
pMD1(avrBs219–714-HA),
pMD1(avrBs2+1fs-HA), or
pMD1(avrBs2+2fs-HA). Symptoms were
photographed 72 h after inoculation.