Fig. 4.

Increased deposition of transmitted α-synuclein by lysosomal failure. Differentiated SH-SY5Y cells were incubated with conditioned medium containing secreted myc-α-synuclein for 2 days. Lysosomal (Baf A1) and proteasomal (MG132) inhibitors were added at the final 16 h of treatment. The cells were immunostained with anti-myc antibody. (A) Fluorescence intensities measured from individual cells. (B) Number of cells with inclusion bodies. Three experiments were performed, and 100 cells were analyzed per sample. Error bars are SEM (*, P < 0.05; **, P < 0.01).