Fig. 5.

Degeneration of primary neurons and mouse cortical neuronal stem cells (MCNSCs) after exposure to the neuronal-cell-derived extracellular α-synuclein. (A) Nuclear fragmentation in the primary cortical neurons after incubation with α-synuclein medium and LacZ medium. Four independent experiments were performed, and on average, 190 cells were analyzed per sample. Error bars are SEM (*, P < 0.05; **, P < 0.01). (B) Quantitative immunofluorescence of activated caspase 3 in the primary cortical neurons after the same incubation as in (A). (C) Caspase 3 activation in the MCNSCs grafted into the Thy-1 α-synuclein transgenic mice. The proportions of the GFP-positive cells that exhibited activated caspase 3 were analyzed at the different time points.