Table 2.

Induction of cerebral β-amyloidosis by implantation of a stainless steel wire

Inoculation	Number of mice with Aβ deposits/total mice
WT-extract-coated wire	0/4
Tg-extract-coated wire	8/8
Tg-extract-coated wire after 10 min at 95 °C	4/4
Tg-extract-coated wire after plasma sterilization	0/4

Stainless steel wires were incubated in Aβ -containing extract from aged transgenic APP23 mice (Tg extract) or age-matched nontransgenic wild-type control mice (WT extract) for 16 h at room temperature and then air-dried. For heat-denaturation, wires were boiled in PBS for 10 min at 95 °C. Plasma sterilization was done in a STERRAD sterilization system. Wires were implanted unilaterally into the hippocampus and overlaying cortex. Four months after implantation, mouse brains were analyzed immunohistologically using an antibody against human Aβ. The number of animals with induced Aβ deposits out of the total number of animals inoculated per group is indicated.