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. 2009 Jul 16;106(31):12664–12669. doi: 10.1073/pnas.0903460106

Fig. 3.

Fig. 3.

Importance of the cleft and rim contacts in stimulating Pol III* and Pol IV replication. (A) Replication activity of the indicated Pol IV mutant proteins was measured as described previously using a filter-binding assay (15, 40). Assays were incubated at 37 °C for the indicated time and contained 10 nM of the indicated Pol IV protein. Results shown represent the average of 2 independent experiments. Error bars represent the range. (B) The ability of the indicated mutant β clamp proteins to stimulate Pol IV+ replication was measured as described above. Replication activity observed with β+ was set equal to 100%. Results shown represent the average of 3 or more independent experiments. Error bars represent the standard deviation. (C) The ability of βR to stimulate Pol III* (5 nM) replication was measured as described above. Results shown represent the average of 3 or more independent experiments. Error bars represent the standard deviation.