Fig. 4.
Presynaptic tachykinin receptors modulate odor-evoked olfactory receptor neuron transmission. Two-photon imaging of ORN synaptic transmission elicited by odor stimulation at high odor concentration (10−1) in control flies and flies expressing dtkr-RNAi in Or83b neurons. (A and B) Two-photon images of the antennal lobe from flies expressing spH in Or83b neurons in control flies (Left) and flies that also express dtkr-RNAi in ORNs (Right). Pseudocolor overlays reveal the change in fluorescence in response in response to (A) ethyl-3-hydroxybutyrate and (B) methyl hexanoate at 2 different optical planes. (C) Ethyl-3-hydroxybutyrate and (D) methyl hexanoate evoked responses quantified as the integrated fluorescence change over time for each glomerulus. Odors were delivered for 2 seconds. (n) 7–8 preparations. t test: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.