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. 2009 Jul 23;106(31):13052–13057. doi: 10.1073/pnas.0906277106

Fig. 1.

Fig. 1.

miR-7 regulates endogenous α-Syn levels. (A) Schematic diagram of human α-Syn mRNA containing the predicted conserved target site of miR-7. The seed match is indicated with the box. (B) miR-7 reduces the level of endogenous α-Syn in HEK293T cells. Cells were transfected with miR-7 precursor at the indicated concentrations and harvested at 48 h after transfection. A representative Western blot using SYN-1 antibody is shown. (C) Mean levels of α-Syn protein in B calculated from 3 independent experiments. (D) Quantitative RT-PCR analysis of α-Syn and EGFR mRNA expression in HEK293T cells. Cells were transfected with 40 nM miR-7 precursor or scrambled sequence and harvested 24 h later. Both mRNA expressions are normalized to GAPDH mRNA and are shown as a ratio to control (scrambled miR-7 sequence)-transfected cells. Experiments were done in triplicates. Data are shown as means ± SD. *, P < 0.01 for difference between control and miR-7-treated samples.