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. 2009 Jun 16;106(31):12873–12878. doi: 10.1073/pnas.0904958106

Fig. 2.

Fig. 2.

NOD2 dependence of cytokine responses to S. aureus. (A and B) Nod2−/− mice (open bars, open circles) and WT mice (closed bars, filled circles) were infected s.c. or i.p. with 5 × 107 cfu of WT S. aureus. IL-6 mRNA levels (normalized to GAPDH) in the infected skin were determined by real-time PCR and are expressed relative to the levels in uninfected WT mice. n = 5 mice/group. IL-6 levels were assayed by ELISA in skin homogenates (n ≥ 4/group) or serum (n ≥ 10/group). All data are mean (SE); *, P < 0.05. (C) Freshly explanted skin of WT mice and Nod2−/− mice was inoculated with S. aureus, and IL-6 levels were determined by ELISA in tissue homogenates 4 h later. Data are mean (SE); n = 4; *, P < 0.05. Bone marrow-derived macrophages from WT mice and Nod2−/− mice were co-cultured with S. aureus (MOI 20) for 24 h. IL-6 levels were determined in supernatants by ELISA. Data are mean (SD) of 1 representative of 3 independent experiments; n = 3; *, P < 0.05.