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. Author manuscript; available in PMC: 2009 Aug 6.
Published in final edited form as: Dev Neurobiol. 2008 Jul;68(8):1076–1092. doi: 10.1002/dneu.20637

Figure 3. Effect of overexpression of HDAC4 lacking the deacetylase domain (HDAC4-N) on neuronal apoptosis.

Figure 3

(A and B) 5 day old cultures of CGNs were transfected with HDAC4 lacking the deacetylase domain (HDAC4-N, Flag tag) or GFP plasmids and switched to HK or LK medium for 24 hrs. Transfected neurons were identified by GFP fluorescence or Flag immunoreactivity and the status of the nuclei assessed by DAPI staining. Panel A quantifies survival of HDAC4-N and GFP transfected cells in HK and LK medium as percent of control (viability of GFP-transfected neurons in HK). P < 0.0003 for comparison with HK-GFP. (B) Subcellular localization of HDAC4-N in HK and LK medium. HDAC4-N (red) is trapped in the nucleus (blue) in both HK and LK conditions.