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. Author manuscript; available in PMC: 2009 Aug 6.
Published in final edited form as: Dev Neurobiol. 2008 Jul;68(8):1076–1092. doi: 10.1002/dneu.20637

Figure 9. Effect of HDAC4 overexpression on cell cycle progression.

Figure 9

(A) HEK293T cells were transfected using GFP and HDAC4 plasmids. Total cell numbers were counted 5 days after transfection using a hematocytometer (P < 0.05).

(B) HEK293T cells were transfected with GFP or HDAC4 plasmids. Protein expression was allowed to persist for 22 hrs after which BrdU was added for an additional 2 hrs. Cells were then harvested and stained for HDAC4, GFP and BrdU. The graph demonstrates percentage of cells expressing protein of interest that are BrdU positive (the data has been normalized to % of GFP expressing BrdU positive cells) (P < 0.002).

(C) Images of HEK293T cells expressing GFP or HDAC4. Shown in blue is DAPI staining of these cells. Shown in red are BrdU positive cells (Texas Red). GFP and HDAC4 overexpressing cells are shown in green (FITC).