Figure 2.
Synthetic thiolactone peptides are biologically active in reconstituted strains. Shown are representative data for activation (A) and inhibition (B) of the agr response by synthetic AIPs. Degree of activity based on β-lactamase activity is shown as a plot of Vinit (initial velocity) vs. peptide concentration. (A) Activation of the agr response in RN9222 (CA1-I) by Group I AIP. The RN9033 (vector alone) control is shown. (B) Inhibition of the agr response in RN9222 (CA1-I) by Group II AIP in the presence of activating Group I AIP at 100 nM.