Figure 6. H3K4me3 and H3K27me3 modifications of key transcription factor genes.
(A) H3K4me3 (blue) and H3K27me3 (red) modifications on Tbx21 (Chr11, 96894000-96939000) and Gata3 (Chr2, 9770000-9830000) loci.
(B,C) Freshly isolated nTreg cells were repolarized under neutral condition (ThN) and Th1 conditions for 72 hours. Proportions of Foxp3- and IFN-γ-producing cells (B) and T-bet (C) were determined by intracellular staining and flow cytometry. The data are representative of two independent experiments.