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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Bone. 2009 Apr 8;45(1):52–60. doi: 10.1016/j.bone.2009.03.675

Figure 3.

Figure 3

Alendronate (50 µM) decreases stress fiber density, focal adhesion density, and focal adhesion area in MC3T3-E1 cells, and effects are antagonized by geranylgeranyl group donor geranylgeraniol (GGOH) (40 µM). Treatments were for 22 hr in FBS medium. A: Confocal micrographs of cells showing actin stress fibers and focal adhesions. Bars represent 25 µm. B–E: Quantification of parameters. Values are means +/− SEM of responses measured in 2 independent experiments in which 40 cells were imaged and quantified per group. Different letters indicate statistically significant differences (95% Tukey confidence interval) from control (a) and between groups (b). There are no statistically significant differences in edge actin bundle (3C), cellular area or circularity (data not shown).