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. 2000 Nov 21;97(24):13342–13347. doi: 10.1073/pnas.97.24.13342

Figure 2.

Figure 2

Induction of L-LTD with 1-Hz stimulation was blocked by inhibitors of translation and transcription. (a) Application of the protein synthesis inhibitor anisomycin (30 μM) (beginning 15 min before the 1-Hz depression protocol until 15 min afterward, horizontal bar) blocked L-LTD for time points subsequent to 40 min (n = 9). (b) When anisomycin application was delayed until 30 min after the 1-Hz depression protocol, there was no effect on L-LTD maintenance (n = 3). (c) Similarly, when the protein synthesis inhibitor emetine (20 μM) was applied (beginning 15 min before the 1-Hz stimulus until 15 min afterward), the induction of L-LTD was prevented for time points later than 40 min (n = 4). Field EPSPs after anisomycin or emetine application were both significantly different from control EPSPs (P < 0.01, measured 1 h after the 1-Hz stimulus). (d) Application of the transcriptional inhibitor actinomycin D (20 μM) (beginning 15 min before the 1-Hz stimulus and continuing until 15 min afterward) blocked L-LTD for time points subsequent to 60 min (n = 6; P < 0.05, as compared with control EPSPs, measured 1 h after the 1-Hz stimulus).