Abstract
The indirect immunoperoxidase technique was assessed for the serodiagnosis of tsutsugamushi fever (scrub typhus). The antigens were peritoneal smears prepared from mice infected intraperitoneally with the Karp, Kato, and Gilliam strains of Rickettsia tsutsugamushi. Treatment of the mice with cyclophosphamide apparently increased the number of the rickettsiae, and it minimized the exudate that interfered with the specific staining. The rickettsiae were seen as clusters in the juxtanuclear region of the mesothelial cells and also as free particles outside of the cells. By the indirect immunoperoxidase technique, the sera from all of the patients (49 samples from 30 patients) were positive for the R. tsutsugamushi antibody. The antibody titers (immunoglobulin G [IgG] and IgM) determined by the indirect immunoperoxidase technique correlated with those determined by the indirect immunofluorescence technique. Thus, the indirect immunoperoxidase technique was useful for quantifying both IgG and IgM antibodies to the rickettsia.
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- BOZEMAN F. M., ELISBERG B. L. Serological diagnosis of scrub typhus by indirect immunofluorescence. Proc Soc Exp Biol Med. 1963 Mar;112:568–573. doi: 10.3181/00379727-112-28107. [DOI] [PubMed] [Google Scholar]
- Crum J. W., Hanchalay S., Eamsila C. New paper enzyme-linked immunosorbent technique compared with microimmunofluorescence for detection of human serum antibodies to Rickettsia tsutsugamushi. J Clin Microbiol. 1980 Jun;11(6):584–588. doi: 10.1128/jcm.11.6.584-588.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dasch G. A., Halle S., Bourgeois A. L. Sensitive microplate enzyme-linked immunosorbent assay for detection of antibodies against the scrub typhus rickettsia, Rickettsia tsutsugamushi. J Clin Microbiol. 1979 Jan;9(1):38–48. doi: 10.1128/jcm.9.1.38-48.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kobayashi Y., Nagai K., Tachibana N. Purification of complement-fixing antigens of Rickettsia orientalis by ether extraction. Am J Trop Med Hyg. 1969 Nov;18(6):942–952. doi: 10.4269/ajtmh.1969.18.942. [DOI] [PubMed] [Google Scholar]
- Shishido A., Hikita M., Sato T., Kohno S. Particulate and soluble antigens of Rickettsia tsutsugamushi in the complement fixation test. J Immunol. 1969 Sep;103(3):480–490. [PubMed] [Google Scholar]
- Tachibana N., Kobayashi V. Effect of cyclophosphamide on the growth of Rickettsia sennetsu in experimentally infected mice. Infect Immun. 1975 Sep;12(3):625–629. doi: 10.1128/iai.12.3.625-629.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]