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. 2009 Jul 14;10:70. doi: 10.1186/1471-2199-10-70

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Copyright © 2009 Sirakov et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RT-qPCR cycle threshold (Ct) values of candidate reference genes among different tissues in two data sets. Cycle threshold distribution values (Ct) of 16S, actB, tubA, EEF1A and ubi (set a, left panel) and 18S, actB, tubA, EEF1A, ubi (set b, right panel) from mantle, supraoesophageal and suboesophageal masses and optic lobes of O. vulgaris. The distribution is shown by vertical box plot as medians (lines), interquartile range (boxes) and ranges (whiskers). Circles mark outliers with values between 1.5 and 3 times the interquartile range; asterisks mark outliers having Ct more than three times the interquartile.