Table 1.
Gene | Transcript | Base pairs | Sense Primer (5'-3') | Antisense Primer (5'-3') |
COL1A2 | NM_000089 | 225 | TCCAAAGGAGAGAGCGGTAA | GCCACTTGCACCACGACTA |
COL4A2 | NM_001846 | 269 | CTGGGTGGCGGAGTTTGTG | GCTGATGTGTGTGCGGATGAG |
COL4A3 | NM_000091 | 157 | CACCAGCTCTGATGCCAATG | AGAGAAATCCAGCCGTGAGG |
DMD | NM_004019 | 151 | GACCAGCACAACCTCAAGCAA | TCAGCAGCCAGTTCAGACACA |
FBXO32 | NM_058229 | 376 | GTCCAAAGAGTCGGCAAGT | TTGGGTAACATCGGACAAGT |
GAPDH | NM_002046 | 172 | TCTGACTTCAACAGCGACAC | TGGTCCAGGGGTCTTACTC |
IGF1 | NM_000618 | 355 | AGCAGTCTTCCAACCCAATTA | CACGGACAGAGCGAGCTG |
IGFBP5 | NM_000599 | 339 | CCAAACACACCCGCATCT | CAGCTTCATCCCGTACTTGTC |
GDF8 | NM_005259 | 167 | TATCACGCTACAACGGAAAC | GGAGTCTCGACGGGTCTC |
MYH1 | NM_005963 | 132 | AAGAGCAGGGAGGTTCACAC | TTATCTCCAAAAGTCATAAGTACA |
NEB | NM_004543 | 131 | CCGTGCCATGTATGACTATAT | CGGTCCTGCCAGTCCTCTG |
PVALB | NM_002854 | 329 | GATGACAGACTTGCTGAACGC | CTTAGCTTTCAGCCACCAGAG |
TRIM63 | NM_032588 | 393 | GAGGATTCCCGTCGAGTGAC | AATGGCTCTCAGGGCGTCT |
Table of primers used for QPCR analysis. Genbank accession number and PCR product length given in parentheses after each transcript name