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Type 1 VHL mutant proteins are unstable. (A) Indicated stably transfected RCC10 cell lines were incubated in media with no cycloheximide (0 time point) or with cycloheximide (100 μg/ml) for the indicated lengths of time. Cell lysates were prepared, equally loaded and subjected to VHL and α-tubulin western blotting. (B) Cycloheximide stability assay as in (A) was performed on the indicated stably infected RCC10 cell lines.
