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Immunohistochemistry for keratin, duct epithelial marker (A-B) and S-100 protein, myoepithelial marker (C-D) in pleomorphic adenoma cell systems: SM-AP1 (A, C), SM-AP4 (B, D) at day 6 after plating, indirect immunofluorescence, × 200. All of the cell systems, SM-AP1 to SM-AP5, were equally immunopositive for keratin (A-B). SM-AP1, SM-AP2, and SM-AP3 cells were not definitely positive for S-100 protein (C), while SM-AP4 and SM-AP5 cells were strongly positive for S-100 protein (D).
