Fig.4.

The effect of MSC medium on the mitochondrial signaling pathway

MSC medium increased the ratio of Bcl-2 (a) to Bax (b) in mitochondria, reduced the release of cytochrome C (c) and AIF (d) into the cytoplasm, and reduced the activation of caspase-3 and cleaved caspase-3 (e). 50 μg of the cytosolic proteins and 20 μg of mitochondrial proteins were analyzed by Western blotting analysis with anti-Bcl-2, anti-Bax, anti-cytochrome C, and anti-AIF. 100 μg of total cell lysates were analyzed with anti-caspase-3. Results are representative of three independent experiments. ^* P<0.05 vs control group, ^# P<0.05 vs DMEM group