Table 1. Primers used in PCR for amplification of MYOC and CYP1B1.
| Exons | Primer sequence (forward/reverse) | Product size (bp) |
|---|---|---|
|
MYOC 1 |
5′-TCACCAAGCCTCTGCAATG-3′ |
654 |
| |
5′-TGAACTCAGAGTCCCCCCAC-3′ |
|
|
MYOC 2 |
5′-GCACCTGCCACCACATCC-3′ |
657 |
| |
5′-ACACATCTCTCATCTTGCCC-3′ |
|
|
MYOC 3 |
5′-GCTGTCACATCTACTGGCTC-3′ |
927 |
| |
5′-ATCTCCTTCTGCCATTGCCT-3′ |
|
|
CYP1B1 2 |
5′-CATTTCTCCAGAGAGTCAGC-3′ |
1260 |
| |
5′-GCTTGCAAACTCAGCATATTC-3′ |
|
|
CYP1B1 3 |
5′-ACCCAATGGAAAAGTCAGCC-3′ |
927 |
| 5′-GCTTGCCTCTTGCTTCTTATT-3′ |
The first column shows the sequenced exons of MYOC and CYP1B1; the second column shows the primer sequences, both forward and reverse ones, used to amplify the corresponding exons indicated in the first column; the third column shows the expected size of the PCR products.