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. 2009 Aug 17;4(8):e6663. doi: 10.1371/journal.pone.0006663

Figure 1. A binary system for in vivo monitoring of mRNA mobility.

Figure 1

(A) System components, reporter mRNA with MS2 binding-sites and GFP-MS2-nls fusion protein. (B–C) Maximum projections of z-series of embryonic muscles: distribution of GFP-MS2-nls in Drosophila myofibers in the absence (B) or presence (C) of the reporter mRNA. Muscles 12 are outlined in white. Circles indicate nuclei containing the GFP-fusion protein. Note heavily labelled nucleoli (*). (C) In the presence of the reporter mRNA, GFP-MS2-nls is observed in the cytoplasm. Nuclei either contain or lack GFP-MS2-nls protein. High levels of expression result in the accumulation of GFP-fusion protein in the nuclei. Arrows indicate empty nuclei. (D, D') DAB-peroxidase immunohistochemistry and immunofluorescence showing the presence of ß-galactosidase in the absence of the GFP-fusion protein. (E–E'') The presence of ß-galactosidase (red) in muscle 12 expressing the GFP-fusion protein (green). E'' shows overlay. Bar (E''), 5 µm.