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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2009 Jul;68(7):785–796. doi: 10.1097/NEN.0b013e3181aaf4fd

Figure 1.

Figure 1

Specificity of leucine-rich repeat kinase-2 (LRRK2) antibodies. 293t cells were transfected with mock cDNA (pcDNA), LRRK2-V5, or LRRK2. Thirty μg of protein of each lysate was resolved in 6% SDS-polyacrylamide and was analyzed by Western blotting using antibodies (A) V5, (B) 1181#2, (C) 1182#1, (D) 2-2E8, (E) 2001#1, or (F) 2002#2. The numbers indicate the polyclonal bleed presented. Representative immunoblots were overexposed to support the specificity of novel LRRK2 antibodies. *Nonspecific immunoreactivity noted only with 1182#1.