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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2009 Jul;68(7):785–796. doi: 10.1097/NEN.0b013e3181aaf4fd

Figure 10.

Figure 10

LRRK2 aggresome formation does not alter the localization of α-synuclein (α-syn). (A) Confocal microscopy of 293t cells co-transfected with LRRK2-V5 and pcDNA3.1/α-syn and treated with MG132 (10 µM, 16 hours). LRRK2 (1181#1; green) formed aggresomes, but α-syn (antibody Syn211, red) remained cytosolic. The image is a single Z-plane of <0.7 µm. Bar scale: 20 µm. (B) 293t cells were transfected with LRRK2-V5 and mock cDNA or pcDNA3.1/α- syn and then treated with MG132. Biochemical cellular fractionation and immunoblotting were performed with anti-V5 or Syn211. LRRK2 redistributed to the insoluble fraction with MG132 treatment, both with and without α-syn co-transfection. α-Syn did not change solubility with MG132 treatment. Data are representative of 3 independent experiments.