Skip to main content
. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2009 Jul;68(7):785–796. doi: 10.1097/NEN.0b013e3181aaf4fd

Figure 6.

Figure 6

Confocal microscopy showing colocalization of LRRK2 with markers of aggresome formation. (A–C) 293t cells were transfected with LRRK2, and (D–F) COS-7 cells were transfected with LRRK2 and treated with MG132 (10 µM, 16 hours) and then double immunofluorescence was performed between aggresome markers anti-γ-tubulin (A, D), antivimentin (B, E), or anti-lamp1 (C, F) and 1181#1. Colocalization was observed for both γ-tubulin and vimentin. Some lamp1 immunofluorescence was noted around the LRRK2 aggregate. All images provided are of a single Z-plane of <0.7 µm. Bar scale: 10 µm.