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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2009 Jul;68(7):785–796. doi: 10.1097/NEN.0b013e3181aaf4fd

Figure 8.

Figure 8

Double immunofluorescence of human brain sections containing pathological α-synuclein (α-syn) inclusions with anti-LRRK2 and anti-α-syn antibodies. Tissue sections from the substantia nigra (A, C–F) from patients with diffuse Lewy body disease (C, E, F) or Lewy body variant of Alzheimer disease (A, D) and cingulate gyrus from a patient with Parkinson disease (B) were examined by double immunofluorescence with anti-LRRK2-specific antibodies (1181#1, 1182#1, 2-2E8, 2001#1, 2002#2) and α-syn-specific antibodies (LB509, Syn505, or SNL4). Antibody 1181#1 (A, red) showed faint immunoreactivity for some rare α-syn-positive Lewy bodies (LB) (arrow) but this immunoreactivity did not exhibit typical LB morphology. 1182#1 (B, red), 2-2E8 (C, green), 2001 (D, red), and 2002 (E, red) demonstrated no immunoreactivity for LBs, Lewy neurites or neuroaxonal spheroids. (F) Control showing that the mouse monoclonal anti-α-syn antibodies LB509 (green) and rabbit polyclonal anti-α-syn SNL4 (red) had identical immunoreactivities. Bar scale: 50 µm.