Table I. Azaspiracid-up-regulated proteins from pool B separated by non-denaturing PAGE.
Underlined amino acids, identical amino acids found in the assigned protein; Oxid, oxidized methionine. The periods denote the site of trypsin cleavage.
| Assignment | Mascot score/number of unique peptides above homology threshold | Peptide sequence identified above homology cutoff and precursor ion mass and charge state for single peptide identifications | Ion scorea | Homology to protein | Method of identification |
|---|---|---|---|---|---|
| Slice B2 | |||||
| SOD | 108/2 | R.LACGVIGISKV.- | 53 | gi 34481600 (M. edulis)b | Nano-LC-ESI-MS/MS |
| K.LSLTGPQSIIGR.T | 55 | ||||
| Murein lipoprotein (major outer membrane lipoprotein) | 59/1 | K.IDQLSSDVQTLNAK.V | 59 | gi 127525b | Nano-LC-ESI-MS/MS |
| 766.39, 2+ | |||||
| Slice B3a | |||||
| SOD | 238/3 | R.LACGVIGISKV.- | 55 | gi 34481600 (M. edulis)b | Nano-LC-ESI-MS/MS |
| K.LSLTGPQSIIGR.T | 67 | ||||
| R.TVVVHADIDDLGK.G | 70 | ||||
| Cathepsin D | 55/2 | K. ISVDGVTPVFFYNMVK.Q | 55 | gi 66560290 (A. mellifera)b | Nano-LC-ESI-MS/MS |
| 834.93, 2+ | |||||
| K. ISVDGVTPVFFYNMVK.Q | 42 | ||||
| Oxid, 842.93, 2+ |
a Score greater than 51 indicates identity, and score greater than 32 indicates homology.
b NCBInr database.