Table II. Azaspiracid-up-regulated proteins from Pool B separated by denaturing SDS-PAGE.

Underlined amino acids, identical amino acids found in the assigned protein; Oxid, oxidized methionine. The periods denote the site of trypsin cleavage.

Assignment	Mascot score/number of unique peptides above homology threshold	Peptide sequence identified above homology cutoff and precursor ion mass and charge state, for single peptide identifications	Ion scorea or E value	Homology to protein	Method of identification
Band nr1
Cathepsin D	68/1	K.EGGELILGGSDPK.H	68a	gi 46309251 (T. pacificus)b	Nano-LC-ESI-MS/MS
		636.32, 2+
	60/2	K.ISVDGVTPVFFYNMVK.Q	60a	gi 66560290 (A. mellifera)b
		834.92, 2+
		K.ISVDGVTPVFFYNMVK.Q	40a
		Oxid, 842.93, 2+
p53 family proteins (DeltaN p63/p73-like protein, p63/p73 like protein, p53 tumor suppressor like-protein)		KKMLLVDER	6.7c	gb AAZ05996.1d	MALDI-TOF/TOF de novo
					novo
		FKGLLDFTDNTPR	7.3c	gb AAZ05995.1d	ESI-MS/MS de novo
		QVSDSATSTAVVEGR	9.7c	gb AAT72301.1 (blue mussel M. edulis)d
Band nr2
SOD	71/1	K.LSLTGPGSIIGR.T	43a	gi 34481600 (blue mussel M. edulis)b	Nano-LC-ESI-MS/MS
		621.37, 2+
Band r3
Glutathione S-transferase Pi		LALLTDWPTDLEATR	13c	gb AAS60226.1 (blue mussel M. edulis)d	ESI-MS/MS de novo
Multidrug resistance-associated protein		NLVELYNDDVDGK	0.41c	gb AAK84397.1d	ESI-MS/MS de novo
		YQWEGGDTRQK	18c	gb AAK84398 (blue mussel M. edulis)d
Band r4
Flagellar P-ring protein precursor		AALMVTADLPPYTPPG	68% identity	A. tumefaciens, Swiss-Prot Q44340 FLGI_AGRT5e	Nano-LC-ESI-MS/MS
		CVTADLPPYPAPGK	40c	M. populi, Ref YP_001923314.1d	ESI-MS/MS de novo
		CVTADLPPYPA	45c
Lysozyme		TLLNDYSAGK	5.5c	gb AAN16207.1 (blue mussel M. edulis)d	ESI-MS/MS de novo
		YGSFWDGHQVK	13c

^a Ion score. Score greater than 51 indicates identity, and score greater than 30 indicates homology.

^b NCBInr database.

^c E value.

^d BLAST.

^e MSBLAST EMBL database.