Figure 1.

Parkin interacts with the E2 ubiquitin-conjugating enzyme, UbcH8. (A) Multiple sequence alignment of ring-finger domains of Parkin (BAA25751), HHARI (NP005735), H7-AP1 (CAB45870), and UIP28 (AAD24572). Conserved residues are highlighted, and the ring fingers (R1 and R2) and the IBR domain are indicated. Abbreviations: HHARI, ariadne homologue (Homo sapiens); H7-AP1, UbcH7-binding protein; UIP28, UbcM4 interacting protein 28. (B) Lysates prepared from HEK 293 cells transfected with pRK5-myc-Parkin and pRK5-HAUbcH5, pRK5-HAUbcH7, or pRK5-HAUbcH8 were subjected to immunoprecipitation (IP) with anti-myc antibody followed by anti-HA immunoblotting. This experiment was replicated three times with similar results.