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. Author manuscript; available in PMC: 2010 Jul 14.
Published in final edited form as: Curr Biol. 2009 Jun 18;19(13):1086–1095. doi: 10.1016/j.cub.2009.05.056

Figure 1.

Figure 1

A compressed mitotic spindle expands asynchronously and reversibly.

(A) Method developed to mechanically perturb spindles in vivo. (B) Fluorescence imaging of a compressed spindle in a Ptk2 EGFP-α-tubulin cell. (C) Time courses of cell and spindle length and width changes upon compression for the cell in (B). (D) Time courses of spindle length and width changes during expansion (31 cells) and contraction (17 cells). For clarity, steady-state time points are not displayed. (E) Fluorescence imaging of a compressed spindle in a Ptk2 EYFP-cdc20 cell, with compression released at 16:30. (B-E), compression started at 0:00 (min:s) and scale bar corresponds to 5 μm.