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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: Cell. 2008 Nov 28;135(5):919–932. doi: 10.1016/j.cell.2008.10.012

Figure 1. A 61-nt small RNA maps near the 3’ end of mMALAT1.

Figure 1

(A) The MALAT1 locus, located on mouse chromosome 19, has been reported to yield a ~7-kb non-coding transcript (transcription start site denoted by arrow) that contains regions of high conservation. MALAT1 lacks repetitive elements except for a SINE and LINE element near its 5’ end. (B) Small RNAs were isolated from EpH4-EV (denoted EV) and EpH4-A6 (denoted A6) cells. Four different probes that span the mMALAT1 locus were used for Northern blot analysis as designated at the bottom of each blot. U6 snRNA was used as a loading control. (C) Strand-specific oligonucleotide probes were used to further map the 61-nt small RNA. Sense probes detect transcripts originating from the opposite strand of mMALAT1, while antisense probes detect transcripts originating from the same strand as mMALAT1. (D) The 61-nt mascRNA transcript is highly conserved (Sequence shown as DNA and does not include CCA at the 3’ end, which is added during processing). (E) The mascRNA transcript lacks translation initiation and stop codons.