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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: Cell. 2008 Nov 28;135(5):919–932. doi: 10.1016/j.cell.2008.10.012

Figure 2. mascRNA is broadly expressed in normal human tissues.

Figure 2

(A) Northern blot analysis using strand-specific oligonucleotide probes showed that the human mascRNA ortholog is expressed in HeLa cells. U6 snRNA was used as a loading control. (B) 10 µg of total RNA from twenty normal human tissues were probed for mascRNA expression. (C) A 25-nt probe complementary to nt 6728–6752 of mMALAT1 was designed. The 25-nt probe specifically distinguished expression of the mouse isoform in EpH4-EV cells from the human isoform in HeLa cells.